NAD+

NAD⁺ (Nicotinamide Adenine Dinucleotide) is a foundational coenzyme present in all living cells, where it plays a central role in oxidation–reduction reactions, ATP production, and cellular repair processes. Purity Base supplies NAD⁺ as a lyophilized powder verified by High-Performance Liquid Chromatography (HPLC) to a purity of ≥99%, making it a reliable reference compound for biochemical and molecular research where consistency is non-negotiable. Its molecular formula is C₂₁H₂₇N₇O₁₄P₂, with a molecular weight of approximately 663.43 Da.

Research background

NAD⁺ has been extensively studied in in vitro and preclinical settings across several areas of cell biology. It is investigated as a key mediator of mitochondrial energy metabolism and ATP homeostasis, and has been referenced in the literature in the context of DNA repair mechanisms and genomic stability. Research has explored its role as a substrate for sirtuin enzymes — a family of NAD⁺-dependent deacetylases implicated in the study of aging-related pathways and metabolic regulation. NAD⁺ is also widely applied as a model compound in oxidative stress research, where the balance between NAD⁺ and its reduced form NADH serves as an indicator of cellular redox state.

Reconstitution & handling

The lyophilized NAD⁺ powder is reconstituted with bacteriostatic water (BAC water). Add an appropriate volume of BAC water directly to the vial, then gently swirl until the powder is fully dissolved. Do not shake the vial, as this may degrade the compound. Handle under clean laboratory conditions and avoid repeated freeze–thaw cycles once reconstituted.

Storage & stability

The lyophilized powder should be stored refrigerated and protected from light and moisture. For extended storage, freezing is recommended. Once reconstituted, the solution should be kept refrigerated at 2–8 °C and used within a short timeframe to preserve integrity. Avoid exposure to heat or prolonged light.

Every order of NAD⁺ from Purity Base is accompanied by a batch-specific Certificate of Analysis (CoA), confirming identity, purity, and HPLC verification data.